JBCS

Soret bands of two states of recombinant orf10-encoded P450-camphor like protein (P450CLA) of Streptomyces clavuligerus expressed in Escherichia coli Rosetta: the resting form (light gray) and the finger print of this hemeprotein characterized by Soret band peaking at 450 nm for the ferrous-CO complex (pink). Cytochrome P450 CLA is involved in the clavulanic acid biosynthetic pathway (color structure at right side) able to inhibit β-lactamase of penicillin-resistant bacteria. See details in the Article Structure and Peroxidase Activity of Ferric Streptomyces clavuligerus orf10-encoded Protein P450CLA: UV-Visible, CD, MCD and EPR Spectroscopic Characterization by Goto et al. on page 913

The inhibition of the enzyme tyrosinase by some new organophosphorus compounds was investigated with the aim of developing potential drugs useful for the treatment of various skin disorders. Some compounds were found to be more active than the commercial ascorbic acid

Sol-gel process used to prepare calcium coating as bioactive materials

There are two chemotypes of "macela" (Egletes viscosa (L.) Less.), whose essential oils are characterized by the presence of trans-pinocarveyl acetate (chemotype A) or cis-isopinocarveyl acetate (chemotype B). Analysis of ITS/5.8S DNA sequences provides an effective and accurate strategy for identifying the chemotypes from E. viscosa

Knoevenagel condensations of aromatic aldehydes with ethyl 4-chloro-3-oxobutanoate were carried out in ionic liquids to give ethyl (E)/(Z)-2-chloroacetyl-3-arylpropenoates. The ionic liquid [bmim(NTf₂)] was reused up to 4 times without loss in product yields, and an unequivocal methodology of (E)/(Z)-structural analysis by ¹³C NMR (nuclear magnetic resonance) is presented

ATR-SEIRAS experiments performed on Pt and PtRu electrodeposits show that CO is formed at low potentials (~ 0.1 V) on both surfaces and remains adsorbed, blocking surface sites and thus inhibiting ethanol oxidation. Kinetic modeling suggests that CO adsorbed layer is not influenced by the presence of Ru, whilst its oxidation is clearly favored in the presence of this metal

The extraction of metal ions present in rice samples with ammoniacal solution of EDTA (pH 10) as extracting agent allowed the determination of P, K, Mg, Ca, Zn, Mn, Cu and Mo in rice samples of type "integral", "agulhinha" and "parboiled"

Bivalves have been widely used as sentinel organisms for environmental monitoring of trace and major element concentration in aquatic ecosystems. The simultaneous characterization of this biomarker in different locations can provide information on the pollution status in the region

A new series of 4-amino-5-aryl-3H-1,2,4-triazole-3-thiones was condensed with different substituted aromatic acids to yield 3,6-disubstituted-1,2,4-triazolo[3,4-b]1,3,4-thiadiazoles. The synthesized compounds were screened for their urease inhibition, antioxidant and antibacterial activities. Some compounds showed excellent urease inhibition activities more than the standard drug

The consumption of cocaine in the Brazilian Federal District, via sewage analysis, was estimated in 1.0 ton year^-1. Sampling at different wastewater treatment plants allowed the detection of areas with higher consumption

The support influences the activity and selectivity of cobalt catalysts on carbon nanotube production. Co/Al₂O₃ showed the highest total carbon yield and the Co/TiO₂ showed the highest selectivity (80%) towards single walled carbon nanotube formation

The synthesis of a series of unstable fluorescent liquid-crystalline arylaldoxime esters 6a-d and 8 is reported. The chemical stability and liquid-crystalline as well as the photophysical properties of the title compounds are described and showed to be dependent on the heating/cooling cycles

Bioassay-guided chemical investigation on the EtOH extract of the peels of Punica granatum led to the isolation of 17 compounds including one new oleanane-type triterpene named punicaone. Flavogallonic acid exhibited the strong fatty acid synthase (FAS) inhibitory activity with IC50 10.3 µmol ^-1

This work presents electrochemical studies, in aprotic media, of twelve Morita-Baylis-Hillman adducts (MBHA) with significant leishmanicidal activity, together with conformational calculations. Ortho adducts present facilitated reduction in comparison to the other isomers and higher leishmanicidal activity. Molecular hardness (N) and atomic charges (QN) values corroborate the obtained experimental data

A variety of heterocyclic systems linked 1-ethylquinolin-2(1H)-one, were prepared from reaction of 3-(nitroacetyl)-1-ethyl-4-hydroxyquinolin-2(1H)-one with some electrophilic and nucleophilic reagents

The ferrous recombinant orf10-encoded P450-camphor like protein (the P450CLA) of Streptomyces clavuligerus exhibits the fingerprint red shift to 450 nm after complexation with CO and peroxidase activity towards tert-butylhydroperoxide

The results indicated that primary sources of vehicular emissions were the dominant source of carboxylic acids in urban and rural PM_2,5 in Londrina City. Moreover, backward trajectories indicated transport of pollutants from Sao Paulo to Londrina

The acid-catalyzed hydrolysis of the glycerol/acetone ketal (solketal) and glycerol/ formaldehyde indicated that solketal was significantly more reactive than glycerol/ formaldehyde acetals. DFT calculations of the ketal and acetal isomers explain the experimental distribution

In the present work, a graphite-polyurethane composite electrode was used for the determination of tetracycline, an antibiotic widely used in animal and human antibacterial treatment. Once in environmental waters, tetracycline can be a potential pollutant from veterinary/medical use as well as in pharmaceutical industry wastes

The figure shows the efficiency of dispersive liquid-liquid microextraction method for the extraction of iron ion with oxine ligand using different extraction (CH₃Cl, CCl₄, CH₂Cl₂ and CS₂) and dispersive (methanol, ethanol, acetone, acetonitrile and THF) solvents

Fluorination treatment improves the discharge capacity and cycle durability of M1Ni_3.5 Co_0.6 Mn_0.4 Al_0.5 alloy, which is attributed to a relatively lower cell volume changes during hydriding/dehydriding. Moreover, the electrochemical reaction resistance of the alloy electrode decreases after the treatment

Prehistoric painting samples from Toca da Extrema II Archaeological Site (Piauí State, Brazil) and Xique-Xique Archaeological Site (Rio Grande do Norte State, Brazil). Score plot of PCA carried on the LIBS (laser-induced breakdown spectroscopy) spectra set of Toca da Extrema II Archaeological Site painting

Environmentally benign and eco-friendly protocol has been developed for the synthesis of 2-amino-3,5-dicarbonitrile-6-thio-pyridine from aldehyde, malononitrile and thiophenol

The official spectrophotometric method (APHA) for quantification of total phenol in water was adapted to flow injection analysis (FIA) for phenol and o-cresol determinations in soil extracts. As shown in the figure, a good agreement between the results was verified in a recovery study, with similar limit of quantification and sampling rate, near 50 analyses per h

Phytochemical study of the aerial parts of Centaurea omphalotricha Coss. & Durieu ex Batt. et Trab., an Algerian endemic plant, afforded three new sesquiterpene lactones together with twelve known compounds, some of them display strong cytotoxic properties in human leukemia cells